Screening Panel (Serology)

Below you will find our screening panel, standard screening programmes, and information about the mouse antibody production (MAP) test.

Agent		Method	Animal Species
1	Mouse hepatitis virus (MHV)	IFA / ELISA	M
2	Rat coronaviruses (SDAV / RCV)	IFA / ELISA	R
3	Reovirus type 3 (Reo3)	IFA / ELISA	M, R, H, Gp, Rb
4	Theiler's murine encephalomyelitis virus (TMEV)	IFA / ELISA	M, R
5	Pneumonia virus of mice (PVM)	IFA / ELISA	M, R, H, Gp, Rb
6	Sendai virus	IFA / ELISA	M, R, H, Gp, Rb
7	Minute virus of mice (MVM)	IFA / HAI	M
8	Mouse parvovirus (MPV)	IFA (+ MVM-HAI)	M
9	Ectromelia virus	IFA	M
10	Lymphocytic choriomeningitis virus (LCMV)	IFA	M, H, Gp
11	Mouse adenovirus (MAd strain K87 and/or FL)	IFA	M, R, Gp
12	Mouse polyomavirus	IFA	M
13	Mouse cytomegalovirus (MCMV)	IFA	M
14	Mouse thymic virus (MTV)	IFA	M
15	Simian virus 5 (SV5)	IFA	H, Gp, Rb
16	Rotavirus	IFA	M, Rb
17	Lactate dehydrogenase-elevating virus (LDV)	LDH enzyme assay	M
18	Hantaviruses	IFA	M, R
19	Kilham rat virus (KRV)	IFA / HAI	R
20	Toolan H-1 virus (H-1)	IFA / HAI	R
20a	Rat parvovirus (RPV)	IFA / HAI	R
20b	Rat minute virus (RMV)	IFA (+ KRV-, H-1-, RPV-HAI)	R
22	Pasteurella pneumotropica	IFA	M, R, H
23	Clostridium piliforme	IFA	M, R, H, Gp, Rb
24	Mycoplasma (M.) pulmonis and/or M. arthritidis	IFA / ELISA	M, R
25	Toxoplasma gondii	IFA	M, R, H, Gp, Rb
26	Encephalitozoon cuniculi	IFA	H, Gp, Rb
27	Treponema cuniculi	TPHA	Rb
28	Myxomatosis virus	IFA	Rb
29	Guinea pig cytomegalovirus (GPCMV)	IFA	Gp
30	Murine norovirus (MNV)	IFA	M
31	Parainfluenza virus type 3 (PIV-3)	IFA	Mee

Abbreviations

• ELISA = Enzyme-linked immunosorbent assay
• Gp = Guinea pig
• H = Hamster
• HAI = Haemagglutination inhibition assay
• IFA = Indirect immunofluorescence assay
• LDH = Lactate dehydrogenase
• M = Mouse
• R = Rat
• Rb = Rabbit
• TPHA = Treponema haemagglutination assay

Standard Screening Programmes

Mouse	Agent no. 1, 4, 5, 6, 7, 8, 16, 22, 23, 24
Rat	Agent no. 2, 5, 6, 19, 20, 20a, 20b, 22, 23, 24
Hamster	Agent no. 6, 10, 22, 23
Guinea pig	Agent no. 6, 11, 26
Rabbit	Agent no. 16, 23, 26

Mouse Antibody Production (MAP) Test

For detecting viral contaminants in biological materials (monoclonal antibodies, cell cultures, hybridoma cell lines, transplantable tumours etc.).

Rat antibody production (RAP) tests can also be performed on request.

MAP tests follow the specifications by Collins und Parker (J. Natl. Cancer Inst. 49:1139-1143, 1972) with special reference to the "Guidelines on the production and quality control of monoclonal antibodies of murine origin intended for use in man." (Commission of the European Communities notes to applicants for marketing authorization. TIBTECH 6:G5-G8, 1988).

One MAP test includes 210 serological tests. All of the 16 viral infections listed in the EC guidelines (plus M. pulmonis) are considered, but not retroviruses. More details can be obtained on request.